Subject area	Biochemistry
More specific subject area	Enzymology
Type of data	Synthetic experimentals/characterization, tables, graphs, figures
How data was acquired	NMR (JEOL ECS-400 400 MHz), IR (Nicolet Avatar FTIR), UV–vis (Shimadzu UV-2450 with TCC-240A cell chamber), HPLC (Agilent 1200 system with degasser, photodiode array detector, and temperature-controlled autosampler)
Data format	Analyzed
Experimental factors	All analytical standards and reagents were confirmed to be >95% purity
Experimental features	The synthesis and characterization of 2,2-diphenylethyl glucosinolate; HPLC standardization of glucosinolate, isothiocyanate, nitrile, and amine analytes; HPLC reaction progress curves for experiments with (1) variable substrate concentration, (2) variable enzyme concentration, (3) variable buffer pH, and (4) variable temperature; tables of initial velocities of hydrolysis/formation
Data source location	Sioux Falls, SD
Data accessibility	The data are available with this article.