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. 2016 Dec 8;4:e2781. doi: 10.7717/peerj.2781

Figure 5. PCR diagnostics.

Figure 5

Comparison of multiplex PCR results using rbcLa universal primers and diagnostic (ARMS) primer. On the left are the results using DNA templates derived from products P1 to P8 with primer specific for bamboo (B), lemongrass (L) and Dianthus (D). Next, fragment pattern predictions (PFP) for presence (+) and absence (−) of corresponding components are depicted. The rbcLa fragment with a size of around 600 bp represents the positive reaction control. A smaller fragment is the diagnostic fragment and indicates the presence of a particular component (e.g., 306 bp fragment for lemongrass). On the right are representative results using DNA templates derived from bamboo (B), lemongrass (L) and Dianthus (D) specimens. For the approximation of fragment size a 100 bp (NEB) size standard (M) was used.