Fig 5.

Western blot analysis of heat shock protein (Hsp)27 and Hsp70. (A) Hsp27 expression in test 1. Note the induction by heat shock at T0 and the reduced expression in group 4 (HS+Do) at T24. (B) Hsp27 in test 2 was more induced by heat shock (group 2) than doxorubicin (group 3). In group 4 at T24 there was an overexpression of the Hsp. (C) Hsp70 expression in test 1. Note the decreased levels in group 4 (HS+Do). (D) Hsp70 expression in test 2. The heat shock induction was more important than in test 1, and there was an overexpression in group 4. Group 1 (control: C); group 2 (heat shock: HS); group 3 (doxorubicin: Do); group 4 (heat shock+doxorubicin: HS+Do). T0: time 0, harvested immediately after doxorubicin treatment. T24: time 24, harvested 24 hours after postdoxorubicin recovery