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. Author manuscript; available in PMC: 2017 Apr 26.
Published in final edited form as: Nature. 2016 Oct 26;540(7632):236–241. doi: 10.1038/nature20165

Extended Data Figure 4. Kinetics of 2HG labelling in 21% and 1% oxygen, by U-13C-glucose and U-13C-glutamine.

Extended Data Figure 4

a/ Proposed mechanism by which HIF-1α controls S-2HG production in CD8+T-lymphocytes and 13C-labelling strategy using U-13C-glucose (m+6) and U-13C-glutamine (m+5) to label endogenous 2HG. Red and green represent pathways promoted and inhibited respectively by HIF-1α in hypoxia.

b/ Isotopologue distribution of 2HG (as a percentage of the total pool) in CD8+ T-lymphocytes, after labelling with U-13C-glucose for 1, 5 and 24 h in both 21% and 1% oxygen conditions; n=3 mice per time point. Error bars denote s.d.

c/ Isotopologue distribution of 2HG (as a percentage of the total pool) in CD8+ T-lymphocytes, after labelling with U-13C-glutamine for 1, 5 and 24 h in both 21% and 1% oxygen conditions; n=3 mice per time point. Error bars denote s.d.