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. Author manuscript; available in PMC: 2017 Apr 26.
Published in final edited form as: Nature. 2016 Oct 26;540(7632):236–241. doi: 10.1038/nature20165

Extended Data Figure 3. Naïve and expanding primary CD8+ T-lymphocytes do not possess mutations in Idh1 or Idh2 that can explain the presence of high levels of 2HG.

Extended Data Figure 3

a/ Illustration outlining the workflow for mutational analysis of Idh1 and Idh2

b/ Sanger sequencing chromatograms validating the presence of wild type Idh1 as compared to the C57BL/6J NCBI reference sequence.

c/ Alignment of mouse and human IDH1 protein indicating conservation of active site arginine residues.

d/ Sanger sequencing chromatograms validating the presence of wild type Idh2 as compared to the C57BL/6J NCBI reference sequence.

e/ Alignment of mouse and human IDH2 protein indicating conservation of active site arginine residues.