Fig 4.

Yeast 2-hybrid interaction test for different sHsps. As prey vectors, we used pADGal4 without insertion (negative control) and with Hsp16.1-CIII as well as its 3HA-tagged form as insertions. The bait vectors (pBDGal4) contained no insert (negative control) or the indicated complementary deoxyribonucleic acid fragments encoding LpHsp17.7-CI, LpHsp17.4-CII, and LpHsp16.1-CIII. Interaction of the fusion proteins was evaluated by growing the yeast strains in 1:100 dilutions on histidine-free media containing the indicated concentrations of 3-amino triazol (3-AT)