Skip to main content
. 2003 Oct;8(4):381–394. doi: 10.1379/1466-1268(2003)008<0381:thsphr>2.0.co;2

Fig 7.

Fig 7.

(A) Sequence comparison of class CIII sHsps from different plants and (B) phylogenetic relationship of nucleocytoplasmic sHsps. (A) Amino acid sequence alignment of LpHsp16.1-CIII with orthologous class CIII sHsps of Arabidopsis thaliana (At), Gossypium arboreum (Ga), Medicago truncatula (Mt), Lactuca sativa (Ls), Triticum aestivum (Ta), Hordeum vulgare (Hv), and Oryza sativa (Os) was done by using the Clustal X 1.8 software. Database accession numbers and number of corresponding expressed sequence tags (ESTs) are LpHsp16.1-CIII, AAK84869 (no EST); AtHsp17.4-CIII, AAD25777 (5 ESTs); GaHsp17.3-CIII, TC11872 (3 ESTs); MtHsp17.1-CIII, TC56172 (3 ESTs); LsHsp17.8-CIII, TC1271 (6 ESTs); TaHsp19.0-CIII, TC41583 (8 ESTs); HvHsp19.1-CIII, TC36830 (4 ESTs); and OsHsp18.6-CIII, OJ1311D08 (8 ESTs). Arrowheads point to the conserved position of introns in the genomic sequences from tomato (63 bp), Arabidopsis (97 bp), and rice (88 bp). Clusters of basic amino acid residues of the nuclear localization signal are shaded, and amino acid residues fitting the β-fold motifs in the α-crystallin domain (β2 to β9) and in the C-terminal extension (β10) are indicated by open arrows. (B) Based on Clustal alignment using the full-length amino acid sequence of the indicated proteins, a tree was drawn and visualized by the TreeView 32 software. The class CIII proteins are clearly clustered on a separate branch distinct from the other 2 classes. Database accession numbers for sequences of class CI and class CII sHsps are AAF79569 (AtHsp17.8-CI), AAD39328 (AtHsp17.6A-CI), AAC95188 (AtHsp17.6B-CI), CAA34208 (AtHsp17.6C-CI), CAA35182 (AtHsp17.4-CI), CAA35183 (AtHsp18.1-CI), CAA45039 (AtHsp17.6-CII), and CAA74399 (AtHsp17.7-CII) for A thaliana; CAA39603 (LeHsp17.8-CI), AAD30454 (LeHsp17.7-CI), LeTC101691 (LeHsp18.1-CI), AAC36312 (LeHsp17.4-CII), and AAC14577 (LeHsp17.6-CII) for Lycopersicon esculentum; AAA33672 (PsHsp18.1-CI) and AAA33670 (PsHsp17.1-CII) for Pisum sativum; and CAA31785 (TaHsp16.9-CI) and CAA41218 (TaHsp17.4-CII) for T aestivum. Accession numbers for class CIII sequences are the same as in (A).