Figure 3.

Dwell times versus force for steps by kinesin-T94S and wild-type kinesin. (A) The dwell time ratio or limp factor for 8-nm steps by the kinesin-T94S mutant (magenta) or wild-type kinesin (green) increases linearly with force, but the increase is significantly greater for the mutant than wild type. (B) The increase in long dwell times of the mutant with increasing force is paralleled by the increase in the average dwell times, while the short dwell times increase more slowly. (C) The long and short dwell times of wild-type increase in parallel with one another and with the average dwell times with increasing force. The error bars show the standard error of the mean (s.e.m.) for the dwell time ratios and average dwell times.