Table 1.
Kinesin-T94S | Wild-type kinesin | |
---|---|---|
kd, ADP release−ATP | 0.00319±0.00079 s−1, n=4, R>0.95 | 0.00154±0.00080 s−1, n=3, R>0.75 |
kd, ADP release+ATP | 0.0128±0.0013 s−1, n=11, R⩾0.90 | 0.00353±0.00017 s−1, n=12, R>0.93 |
kd, ADP release+MTs | 0.0287±0.0187 s−1, n=9, R⩾0.73 | 0.0162±0.0062 s−1, n=8, R⩾0.65 |
Velocity, MT gliding | 255±13 nm/s, n=20 | 853±25 nm/s, n=19 |
Values are the mean±s.e.m.; R, linear correlation coefficient for the curve fit (values closer to 1.00 indicate a better fit); MT, microtubules. |