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. 2004 Jul 8;23(15):3061–3071. doi: 10.1038/sj.emboj.7600309

Figure 3.

Figure 3

Raf activation can regulate the expression of transcription factors associated with Schwann cell differentiation. (A) Diagram summarising the expression patterns of the transcription factors Oct-6 and Krox-20, and the myelin sheath proteins, represented by P0, during Schwann cell differentiation. (B) Western blot analysis and (C) semiquantitative RT–PCR analysis of NSΔRafER cells treated with either control solvents (C), 1 mM db-cAMP (D), Tmx, or db-cAMP and Tmx simultaneously (D+Tmx) for 48 h. A second set of NSΔRafER cells were treated with db-cAMP or control solvents for 72 h, then Tmx (D->D+Tmx) was added, where stated, for 48 h while maintaining the samples C and D for 120 h in total. Samples were analysed for the effects of Raf activation on the expression patterns of Krox-20 and Oct-6.