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. 2004 Jul 8;23(15):3061–3071. doi: 10.1038/sj.emboj.7600309

Figure 4.

Figure 4

Raf signals via the ERK pathway to block or reverse Schwann cell differentiation. NSΔRafER cells in defined medium were treated with control solvents (C), db-cAMP (D), Tmx, or db-cAMP and Tmx (D+Tmx) for 48 h in the presence or absence of the MEK inhibitors U0126 (30 μM) (Ai) or PD184352 (1 μM) (B). Lysates were analysed by Western blotting with the specified antibodies. (Aii) NSΔRafER cells were treated with control solvents (C) or db-cAMP (D) for 72 h. U0126 was then added to one set of dishes with fresh control solvent or db-cAMP, and with Tmx (D->D+Tmx) where stated for 48 h. (C) NSΔRafER cells in defined medium were treated with the specified dose of U0126 (μM) for 48 h.