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Poly(A) sizing assay for various mutants. Total RNA from various strains was 3′-end-labeled with [32P]-pCp, digested, and then separated on a 20% polyacrylamide gel as described in the Experimental Procedures. The genotype of each strain is listed above each lane. Lane 1, A 5′-[32P]-end-labeled oligo(A) ladder (nt).
