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. 2004 Jul 1;23(14):2892–2902. doi: 10.1038/sj.emboj.7600278

Figure 5.

Figure 5

Postnatal laminar defects restricted to deep CP layers in Ngn2 mutants. At P7, layer VI expression of Slit1 (A, B) and Tbr1 (C, D) was severely reduced in the rostral cortex of Ngn2 mutants (asterisks, B, D). Layer V expression of Robo1 (E, F) and ER81 (G, H) demarcated a thinner, disorganized layer, and ectopic expression in the IZ of Ngn2 mutants (asterisks, H). Layer IV expression of RORβ at P7 (I, J), layer II/III/V expression of Oct6 at P5 (K, L) and layer II/III expression of Id2 (rostral limit marked by arrowhead) at P5 (M, N) were unaffected in Ngn2 mutants (J, L, N). Id2 expression was lost in Ngn2 mutant layer VI (asterisks, N). (O, P) Nissl staining of P5 wild-type (O, O′) and Ngn2 mutant (P, P′) cortices revealed no defects in cortical layering, but neurogenesis defects were evident in the SP (*) and dentate gyrus (**; P) of Ngn2 mutants. (Q, R) P12 cortices stained for synaptic zinc, a marker of axon terminals of glutamatergic projection neurons in layers II/III and VI, were normally excluded by layer IV in P12 Ngn2 mutants (R). gz, germinal zone; h, hippocampus; dg, dentate gyrus; sp, subplate; ALBSF, antero-lateral barrel subfield; PMBSF, posterio-medial barrel subfield; Hp, hindpaw; Fp, forepaw; Lj, lower jaw; SII, secondary somatosensory cortex; Aud, primary auditory cortex; V1, primary visual cortex.