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. 2004 Jun 24;23(14):2713–2722. doi: 10.1038/sj.emboj.7600280

Figure 4.

Figure 4

Model of a gelsolin-capped filament. (A) The ADP model of the actin filament (four protomers are drawn in blue and gray), with G1–G3:actin (1RGI) and G4–G6:actin (G4, pink; G5, dark green; G6, orange; PDB 1H1V; Choe et al, 2002) overlaid onto the barbed end. Purple spheres mark Asp371 of G3 and Met412 of G4, a gap of 63.1 Å to be bridged by the G3–G4 linker, which is modeled in purple. (B) A second view of the gelsolin-capped model filament, looking directly at the capped, barbed end. Three actin protomers are depicted. (C) The G1:actin structure (red:blue) with subdomain 2 of a second actin protomer from the ADP model drawn in black. The arrow indicates a steric clash between the long helix of G1 and the ADP helix of actin subdomain 2. (D) The tail latch. G2 (green) and the C-terminal tail (residues 736–755; orange) from inactive gelsolin positioned on the ADP model by overlaying G2 in (A). Only one actin from the filament is shown (gray). In this position, the C-terminal tail obscures the actin-binding site on G2.