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Transfection efficiency of lPEI/DNA nanoparticles prepared by FNC and bulk mixing methods in different organs of Balb/c mice at 48 h following i.v. injection. lPEI/DNA complex nanoparticles were prepared with lPEI polymer solutions at pH 3.5 for both methods. DNA concentration was 25 μg/mL. The flow rate was 20 mL/min for FNC. Each bar represents mean ± standard deviation (n = 4).
