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. 2016 Dec 12;7:1987. doi: 10.3389/fmicb.2016.01987

FIGURE 3.

FIGURE 3

The intradermal inoculation pathway induces a strong local inflammatory response in CLN. C57BL/6 mice (n = 5 per group) were infected using the routes of infection described in the methods section. At 8 and 29 days post-infection, mice were sacrificed and organs were weighed and analyzed for their bacterial loads by plating homogenates on nutrient agar. Data represent mean of CLN (Left panel) and spleen (Right panel) weight (A) or CFU per organ (B) from three independent experiments. Analysis significance was determined using ANOVA (∗p < 0.05; ∗∗p < 0.005; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001). (C) C57BL/6 mice (n = 5 per group) were infected using different routes of infection as described in methods section. At 8 and 29 days post-infection, total RNA of the CLN was extracted and analyzed for inflammatory response gene expression by reverse transcription real-time PCR. Results are given as fold increase compared to the signal obtained for mock-infected mice. Statistical significance was determined using unpaired, two-tailed Student’s t-test (Results of three independent experiments) (∗p < 0.05; ∗∗p < 0.005; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001) and &significant compared to the oral route; #significant compared to the intranasal route and ‡significant compared to the conjunctival route using ANOVA.