FIGURE 4.

Inhibition of SOCE by the external cations, Gd³⁺, Zn²⁺, and Cu^+/2+. The protocol used is the same as in Figure 2. (A) The Tg-evoked entry of Ca²⁺ was recorded in the absence (open circles, n = 102 cells) or presence of various concentrations of GdCl₃ (filled symbols; 10 nM n = 144 cells; 50 nM n = 132 cells; 500 nM n = 112 cells; 5000 nM n = 67 cells). (B) dose-response curve showing the inhibition (%) of the peak amplitude of the SOCE as a function of the external concentration of GdCl₃. ^∗ and ^∗∗, p < 0.05 and p < 0.01, respectively. (C) Inhibitory effect of 10 μM ZnCl₂ (open squares, n = 129 cells) and 10 μM CuCl₂ (gray triangles, n = 115 cells) on SOCE. Filled circles: untreated (control) cells (n = 101 cells). The graphs (A,C) show the last 200 s of the recording. Mean ± SEM.