Fig. 3.

ANO1 as possible target of miR-132. a MiR-132 expression markedly suppressed ANO1 mRNA levels. b MiR-132 inhibitor resulted in up-regulation of ANO1 mRNA levels. c The 3′ UTR of ANO1 mRNA contains site for seed region of miR-132. d Activity of luciferase reporter containing predicted miR-132 binding sequence of ANO1 3′ UTR was significantly repressed by ectopic expression of miR-132. e ANO1 expression was higher in primary CRC with liver metastasis and in liver metastases than in primary tumors without metastasis (P = 0.0059 and P = 0.0001, respectively). f Significant inverse correlation was observed between miR-132 expression and ANO1 expression in validation cohort. Data are presented as mean ± SD. NC negative control-transfected cells, mimic miR-132 miR-132-transfected cells, inhibitor miR-132 inhibitor miR-132-transfected cells. *P < 0.05