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. 2004 Jul 1;23(14):2853–2861. doi: 10.1038/sj.emboj.7600299

Figure 4.

Figure 4

DHS-mediated regulation requires MSL proteins but not roX RNA. (A) Quantitative real-time RT–PCR of ProX1-DHS males (M) or females (F) in wild-type or different msl or roX1roX2 mutant backgrounds (as indicated at the bottom). Results are represented as in Figure 3D, with the transcription level of wild-type female designated as 1. (B) Left panel: in situ hybridization of roX1 RNA (red) in msl3 [Hsp83-MSL2] females. No roX1 RNA was detected on X. We also failed to detect transcription at the endogenous roX1 locus (3F), as indicated by the arrow. Right panel: in the presence of an Hsp83-roX1 transgene on an autosome, roX1 RNA was detected at many CESs on the X chromosome. (C) Polytene chromosomes from ProX1-DHS-1 stained with anti-MSL1 antibody (red). The top panel shows the male chromosomes in a wild-type background; the bottom panel shows the chromosomes from females with ectopic MSL2 protein but mutant for roX1 and roX2. The arrows indicate the transgene loci. X: X chromosome. DNA was stained with DAPI (blue) in (B, C).