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Native human GrA potentiates LPS-induced tumor necrosis factor-α(TNFα) release from monocytes. Human monocytes were incubated with isolated native human GrA (500 nM) or recombinant GrA (500 nM) with or without LPS (5 ng/ml) for 6 h. TNFα levels in the culture supernatants were determined. Data are expressed as mean±S.D. and are representative of at least three independent experiments with normal donors (*P<0.05, compared with LPS control).
