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. 2016 Nov 28;2016:9014364. doi: 10.1155/2016/9014364

Figure 3.

Figure 3

TM caused apoptotic alternations in mitochondrial function. (a) TM caused intracellular ROS accumulation in HepG2 and SMMC-7721 cells analyzing via DCFH-DA staining after 6 h incubation (20x; scale bar: 100 μm) (n = 3). (b) TM induced MMP dissipation detecting by JC-1 staining after 6 h incubation (20x; scale bar: 100 μm) (n = 3). (c) 24 h TM exposure enhanced the expressions of cleaved-PARP, Bax, and Bad and suppressed the levels of Bcl-2. Quantification data of protein expressions were normalized by corresponding GAPDH and the average fold of band intensity was marked, respectively (n = 3).