FIG. 2.

Analysis of binding of soluble α_Vβ₃ and α_Vβ₆ to FMDV. (a and b) Virus (1 μg) was captured on antibody-coated plastic wells as described in Materials and Methods. Culture medium from nontransfected HEK 293A cells (grey bars) or from transfected cells containing either soluble α_Vβ₆ (a) or α_Vβ₃ (b) (0.5 μg/well; black bars) was incubated with the virus, and binding of integrin was determined using either rabbit anti-α_Vβ₃ (a) or MAb CSβ6 (b) as described in Materials and Methods. (c) Wells were coated with either medium from nontransfected cells (grey bars) or from transfected cells containing soluble α_Vβ₃ (1 μg/ml; black bars) as described in Materials and Methods. Virus (1 μg) was incubated with the integrin, and the binding of virus was determined using an antiviral MAb.