FIG. 3.
Effect of soluble integrins on the adsorption of FMDV to cells. FMDV types A12 and O1C3056H, labeled with [35S]methionine and purified as described in Materials and Methods, were incubated either with soluble αVβ3 or αVβ6 at the concentrations indicated in the figure or with medium from nontransfected HEK 293A cells. Binding assays were performed in BHK3αVβ6 cells at 4°C, using a virus concentration of 3,000 particles/cell, as described in Materials and Methods. (a and b) Soluble αVβ6 incubated with either type A12 (a) or O1C3056H (b). (c and d) Soluble αVβ3 incubated with either type A12 (c) or O1C3056H (d).