FIG. 4.

Analysis of the virus-soluble α_Vβ₆ complex. The samples used for these binding assays were centrifuged in an SW41 rotor through a 10-to-50% (wt/vol) sucrose gradient in TBS+ at 17,000 rpm for 18 h at 4°C. The gradients were fractionated into 0.4-ml fractions, and 0.1-ml samples were counted. (a) [³⁵S]methionine-labeled type A₁₂ incubated with either medium from nontransfected cells (•) or soluble α_Vβ₆ (6 μg/ml) (○). (b) [³⁵S]methionine-labeled type O₁C3056H incubated with either medium from nontransfected cells (•) or soluble α_Vβ₆ (6 μg/ml) (○). Samples were run on parallel gradients but are represented on the same graph for clarity.