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. 2004 Sep;78(18):10197–10201. doi: 10.1128/JVI.78.18.10197-10201.2004

FIG. 5.

FIG. 5.

The R75T variation does not impair HIV-1 SF2-Nef function. (A) P4-CCR5 cells were infected in triplicate with two independent virus stocks of the indicated X4- or R5-tropic HIV-1 Nef variants containing 1.0 ng p24 antigen. Shown are average values of the 6 measurements ± standard deviations. Infectivity is shown relative to the respective HIV-1 NL4-3 variant expressing the wild type T71-Nef. (B) Unstimulated PBMC were infected in duplicate with the X4-tropic HIV-1 Nef variants and cultured as described in the legend to Fig. 2. Similar results were obtained with an independent virus stock and with PBMC derived from two different donors. (C) Replication of R5-tropic HIV-1 SF2 R75- and T75 Nef variants in cocultures of imDCs and autologous PBMC. Cells were isolated, characterized and infected as described in the legend to Fig. 3. The results were confirmed in two independent infections. (D) Replication of X4-tropic HIV-1 NL4-3 Nef variants in ex vivo HLT. Infections were performed as described in the legend to Fig. 4 and virus production was monitored by RT assay.