TABLE 2.
Virus titers of reverse genetic viruses in various organs of chickens
| Day after infection | Virusa | Viral titers in organs harvested from infected chickens (log10 EID50/ml)b
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Brain | Thymus | Liver | Spleen | Pancreas | Kidney | Lung | Bursa of Fabricius | ||
| 1 | rg HP | 3.75 | 4.25 | 2.50 | 4.75 | 3.75 | 3.25 | 6.25 | 4.50 |
| rg HAMP, NAHP | 3.50 | 3.25 | 2.50 | 4.50 | 3.50 | 2.75 | 4.50 | 4.00 | |
| rg HAHP, NAMP | 3.75 | 4.25 | 2.50 | 4.75 | 3.50 | 3.00 | 6.00 | 4.50 | |
| rg HAMP, NAMP | 3.50 | 2.50 | 2.00 | 4.50 | 3.50 | 2.75 | 4.75 | 3.75 | |
| 2 | rg HP | 5.00 | 4.25 | 2.00 | 2.25 | 1.00 | 3.25 | 6.25 | 4.50 |
| rg HAMP, NAHP | 3.50 | 3.50 | < | < | < | 1.00 | 5.00 | 3.50 | |
| rg HAHP, NAMP | 4.75 | 3.50 | < | < | 1.00 | 4.50 | 5.25 | 4.75 | |
| rg HAMP, NAMP | 3.75 | 3.75 | 1.00 | < | < | < | 5.25 | 3.75 | |
| 3c | rg HAMP, NAHP | 2.00 | 1.50 | 1.00 | 2.50 | 1.00 | 2.25 | 4.75 | 3.50 |
| rg HAHP, NAMP | 4.00 | 3.75 | < | < | < | 3.25 | 4.25 | 4.25 | |
| rg HAMP, NAMP | 1.50 | 1.00 | < | < | < | < | 3.50 | 2.00 | |
| 4d | rg HAMP, NAHP | 1.00 | 1.00 | < | < | < | < | 4.00 | 3.00 |
| rg HAMP, NAMP | 1.00 | < | < | 1.00 | < | 1.00 | 2.50 | 2.25 | |
| 5 | rg HAMP, NAHP | < | < | < | < | < | < | 4.75 | < |
| rg HAMP, NAMP | < | < | < | < | < | < | < | < | |
| 6e | rg HAMP, NAHP | < | < | < | < | < | < | 2.25 | < |
| rg HAMP, NAMP | < | < | < | < | < | < | < | < | |
Chickens were infected intravenously with 100.5 EID50 of the virus listed. rg, reverse-genetic virus; HP, highly pathogenic A/chicken/Hong Kong/YU562/01 (H5N1); MP, moderately pathogenic A/goose/Hong Kong/437-10/99 (H5N1). All viruses contain the internal proteins (PB2, PB1, PA, NP, M, and NS) of the HP virus.
Titers displayed are the log10 EID50 per milliliter of tissue homogenate. <, titer below level of detection.
All of the chickens infected with the reverse-genetic HP virus were dead by day 3 after infection.
All of the chickens infected with the reverse-genetic HAHP, NAMP virus were dead by day 4 after infection.
One chicken infected with the reverse-genetic HAMP, NAHP virus died on day 5 after infection, leaving no chickens for sampling of organs on day 7 after infection. No virus was detected in chickens inoculated with the HAMP, NAMP virus on day 7 after infection.