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. 2004 Sep;78(18):9936–9946. doi: 10.1128/JVI.78.18.9936-9946.2004

FIG. 2.

FIG. 2.

Western blot analysis of the TR(+)Sepharose bound and unbound fractions of BC3 and BJAB cell extracts. (A) Comparison of factors bound to the TR(+)Sepharose column. An aliquot of cell extract (CE; 0.1%), pass-through (Th; 0.01%), and KCl-eluted fractions of BC3 and BJAB cells were separated by SDS-4 to 20% PAGE, blotted on a polyvinylidene difluoride membrane, and analyzed with the commercially available antibodies shown to the left of the panel (α, anti). Typically detected bands are indicated by a bracket for LANA and arrows for ORC2, CDC6, Mcm7, and MSH6. (B) Comparison of bound and unbound fractions to TR(+)Sepharose and K-oriLyt CS Sepharose. K-oriLyt CS was also tested for TPA-uninduced or -induced materials. Binding of PARP1 and MSH6 to TR and K-oriLyt CS was examined. +, present; −, absent.