FIG. 6.

Colocalization of replicase proteins and cellular proteins in SARS-CoV-infected Vero Cells. Vero cells on glass coverslips were infected with SARS-CoV (Urbani strain) at an MOI of 1.0 TCID₅₀ per cell. At 6 h p.i., cells were fixed with 100% methanol and processed for dual labeling using guinea pig anti-nsp8 (red, Alexa 546 in all panels) and rabbit anti-nsp3, anti-nsp 2, or anti-LC3 (green, Alexa 488). (A) Colocalization of nsp8 (red) and nsp3 (green). (B) Magnification of panel A cells (white outline) to show single-cell detail. (C) Colocalization of nsp8 (red) and nsp2 (green). (D) Colocalization of nsp8 (red) and LC3 (green). See the text for details. The merged column shows colocalization of red and green signal as yellow pixels. Images were obtained on a Zeiss LSM 510 confocal microscope.