TABLE 1.
SARS-CoV replicase antibodies
| Proteina | Predicted amino acids | Cloned nucleotides | Amino acids | Antiserum (rabbit) | Useb | Predicted/observed mass (kDa) |
|---|---|---|---|---|---|---|
| nsp1 | M1-180G | 265-945 | 1-227 | VU231 | IB/IF | 20/20 |
| nsp2 | A181-818G | 946-2718 | 228-818 | VU239 | IB/IF | 70/70 |
| nsp3 (PLP) | A819-2740G | 2719-3726 | 819-1154 | VU233 | IB/IF | 213/213 |
| nsp4 | K2741-3240Q | 8572-9235 | 2770-2990 | VU237 | IB/IF | 56/38 |
| nsp5 (3CLpro) | S3241-3546Q | 9986-10590 | 3241-3442 | VU261 | IB | 33/30 |
| nsp8 | A3920-4117Q | 12022-12615 | 3920-4117 | VU249 | IB/IF | 22/22 |
| nsp9 | N4118-4230Q | 12616-12954 | 4118-4230 | VU245 | IB/IF | 12/12 |
| nsp12 (PoI) | S4370-5301Q | 14334-15266 | 4690-5001 | VU257 | IB | 106/106 |
| nsp13 (Hel) | A5302-5902Q | 17053-17969 | 5597-5902 | VU253 | IB/IF | 67/67 |
| nsp14 (ExoN) | A5903-6429Q | 18600-19444 | 6112-6393 | VU265 | IB | 60/60 |
| nsp15 (XendoU) | S6430-6775Q | 19746-20425 | 6494-6720 | VU263 | IB | 38/38 |
| nsp16 (2′-O-MT) | A6776-7073N | 20674-21483 | 6804-7073 | VU251 | IB | 33/33 |
a
Hel, helicase; ExoN, exonuclease of DEDD superfamily; XendoU, poly(U)-specific endoribonuclease; 2′-O-MT, 2′-O-methyltransferase.
b
IB, immunoblot; IF, immunofluorescence.