Figure 2.

DNA synthesis in IPEC-J2 cells. DNA synthesis during the proliferation of IPEC-J2 cells was quantified by EdU incorporation (red color) using Cell-Light™ EdU Kit (Rui Bo Biotechnology Limited Company, Guangzhou, China). Nuclei are shown in blue color. Cells were treated with 0 (NC) or 800 μM NAC and 0 or 100 μM H₂O₂, respectively. (a) The percentage of EdU-positive cells (the number of red nuclei versus the number of blue nuclei in at least five different microscopic fields of vision). (b) Representative images of EdU staining (magnification ×200) of cells. Data are expressed as means ± SEM of at least three independent experiments. ^a–cValues with different letters are significantly different (P < 0.05).