Figure 3.

Mitochondrial respiration of IPEC-J2 cells measured by the XF-24 Extracellular Flux Analyzer and Cell Mito Stress Test Kit from Seahorse Biosciences (North Billerica, MA, USA). (a) Schematic and (b) oxygen consumption rate (OCR) assessed by extracellular flux analysis. OCR was measured under basal conditions followed by the sequential addition of oligomycin (0.5 μM), FCCP (1 μM), rotenone (1 μM), or antimycin A (1 μM). Each data point represents an OCR measurement. (c) Individual parameters for basal respiration, proton leak, maximal respiration, spare respiratory capacity, nonmitochondrial respiration, and ATP production were determined. Cells were treated with 0 (NC) or 800 μM NAC and 0 or 100 μM H₂O₂, respectively. Data were expressed as means ± SEM of at least three independent experiments. ^a–cValues with different letters are significantly different (P < 0.05).