Figure 4.

The TCA cycle intermediates, pyruvic acid, and lactic acid of IPEC-J2 cells measured by an Agilent 7890B-5977A GC-MS equipped with HP-5ms (30 m × 250 μm × 0.25 μm) capillary column (Agilent J&W, Santa Clara, CA, USA). Cells were treated with 0 (NC) or 800 μM NAC and 0 or 100 μM H₂O₂, respectively. Data were expressed as means ± SEM of at least three independent experiments. ^a–cValues with different letters are significantly different (P < 0.05).