TABLE 2.

Nucleotide and amino substitutions observed in the predominant circulating virus from serum samples from Ch1536^a

HCV region	NT	NT changeb	AA	AA changeb	Substitutionc at wk:
					22	60	130	216
C	845	C-T					Mixede
E1	1043	C-T						x
E1	1134	C-A	265	L-I	X	X	X	X
E2	1540	T-C	400	V-A		X	X	X
E2d	1548	C-T	403	L-F		Mixede	X	X
E2	1673	G-C	444	Q-H			X
E2d	1728	A-C	463	T-A	X	X	X	X
E2	1910	C-T						Mixede
E2	1923	A-G	528	S-G			X	X
E2	1941	A-G	534	T-A				X
E2	1997	T-C						x
E2	2029	T-C	563	V-A			X	X
E2d	2067	A-G	576	N-D				X
E2	2468	C-T				x	x	x
p7d	2689	T-C	783	V-A				X
p7	2718	G-A	793	M-V		X	X	X
NS2	2843	T-C						x
NS2	2927	C-T						Mixede
NS2	2994	G-A	885	V-I				X
NS2	3047	T-C				x	x	x
NS3	3883	G-A	1181	R-K	X	X	X	X
NS3	4825	A-G	1495	K-R				Mixede
NS3	4892	C-T						Mixede
NS3	5042	T-C				x	x	x
NS4A	5357	G-T						Mixede
NS4A	5424	A-G	1695	I-V				X
NS4B	5984	T-C						x
NS4B	6245	T-C						x
NS5A	6484	G-C	2048	G-A				X
NS5A	6533	C-T						x
NS5A	7083	A-G	2248	N-D				X
NS5A	7382	T-C				x	x	x
NS5A	7386	A-G	2349	T-A		X	X	X
NS5A	7575	G-A	2412	A-T		X	X	X
NS5B	7698	C-A	2453	H-N	X	X	X	X
NS5B	7707	C-A	2456	L-M		X	X	X
NS5B	7715	T-C						x
NS5B	8004	G-A	2555	D-N		X	X	X
NS5B	8576	A-G						x
NS5B	8846	T-C						x
NS5B	9135	C-T				x	x	x
NS5B	9193	G-A	2951	R-L				X
STOP	9376	G-A	TGA	TAA		x	x	x

^aNonsynonymous mutations are shown in boldface type. NT, nucleotide; AA, amino acid.

^bNT and AA changes are shown with the original NT or AA before the hyphen and with the new NT or AA after the hyphen.

^cSubstitutions observed at weeks 22, 60, 130, and 216 p.i. The presence of a synonymous (x) or nonsynonymous (X) substitution is indicated.

^dLocated in previously identified human or chimpanzee T-cell epitopes.

^e“Mixed” indicates that both the original and new NT sequences were observed as mixed peaks on the electropherogram.