FIG. 2.

Western blot analysis of total histone proteins present in Sy5y cells during an HSV-1 infection. (A) Sy5y cells were mock infected or infected with HSV-1 and harvested at the times (in hours) p.i. indicated. Antibodies detecting unmodified histone H3 or the covalently modified amino-terminal tail of histone H3 are indicated. Each histone modification was assayed in at least two independent infections with essentially similar results, and a representative experiment is shown. (B) Quantitation of Western blots presented as the percent change of the HSV-1 samples compared to mock-infected samples (set at 100%) at the same time points.