FIG. 1.

The Us11 gene product is required for translation of viral proteins in primary human fibroblasts treated with IFN-α. FS4 cells untreated or treated overnight with 500 U of recombinant human IFN-α/ml were mock infected or infected (MOI = 5) with either a γ₁34.5 deletion mutant (Δ34.5), a Δ34.5 strain that expressed Us11 as an IE protein [Δ34.5-(IE)Us11], a Us11 null mutant (pAUs11), a virus in which the Us11 mutant allele was repaired (pAUs11-Rep), or WT HSV-1 (WT). At 18 h postinfection, the cultures were metabolically labeled with ³⁵S-amino acids for 1 h. Total protein was isolated and fractionated by SDS-polyacrylamide gel electrophoresis. An exposure of the fixed, dried gel is shown. The bottom panel shows an immunoblot of the samples that was probed with a polyclonal antibody raised against the γ₁34.5 protein. The arrowhead to the right of the blot denotes the position of the full-length γ₁34.5 polypeptide encoded by the HSV-1 Patton strain. The migration of molecular mass standards (in kilodaltons) appears to the left of each panel.