FIG. 2.

The inhibition of translation in IFN-treated cells requires entry into the late phase of the viral life cycle. (A) FS4 cells treated with PAA in the presence and absence of IFN-α were infected with the indicated viruses, metabolically labeled with ³⁵S-amino acids for 1 h at 14 h postinfection, and processed as described in the legend to Fig. 1. In the lower panel, samples were analyzed by immunoblotting with anti-PKR antisera. (B) FS4 cells, either untreated or treated with PAA, were infected with the indicated viruses and processed as described in the legend to Fig. 1. The lower panel shows an immunoblot of the samples that was probed with an anti-gC polyclonal antibody.