FIG. 1.

Fusion activity and cleavage of NiV F protein in different cell lines. (A) Vero, HeLa, and MDCK cells were infected at an MOI of 0.1. At 72 h postinfection, syncytium formation was visualized by staining with Giemsa staining solution. Magnification, ×100. (B) Vero, HeLa, MDCK, and 293 cells were cotransfected with the NiV G and F genes. At 24 h posttransfection, cells were fixed and incubated with Giemsa staining solution. Magnification, ×100. (C) At 24 h posttransfection, NiV F-expressing Vero, HeLa, MDCK, and 293 cells were surface labeled with biotin and were lysed. Following immunoprecipitation, samples were subjected to SDS-PAGE under reducing conditions and were blotted to nitrocellulose. Surface-labeled F proteins were visualized with streptavidin-peroxidase and chemiluminescence.