FIG. 6.
Proteolytic processing and fusion activity of NiV FL110R and NiV FL110F. (A) Cells transfected with either parental F (NiV F), mutant FL110R (NiV FL110R), or FL110F gene (NiV FL110F) were surface biotinylated. F proteins were immunoprecipitated and detected as described in the legend to Fig. 1. (B) At 24 h after cotransfection of the NiV G gene with parental F (NiV F+G), mutant FL110R (NiV FL110R+G), or the FL110F (NiV FL110F+G) gene, cell-to-cell fusion was visualized by Giemsa staining. Magnification, ×100.