FIG. 1.

Specificity of the CsPP2 antiserum. (A) SDS-PAGE and Coomassie blue staining of phloem exudate from different pumpkin cultivars (lanes 1 to 5: cv. Romani, cv. Tanda chiaro, cv. Virginia, cv. Black beauty, and cv. Argelia temprano, respectively) and cucumber (lane 6). The arrowhead indicates the position at which the different PP2s migrate. (B) The proteins were transferred to a PVDF membrane and incubated with the polyclonal CsPP2 antiserum. CsPP2 was detected, and cross-reaction was not observed with the pumpkin PP2s. Standard marker proteins (in kilodaltons) are indicated (lane MW).