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. 2004 Sep;78(18):9890–9903. doi: 10.1128/JVI.78.18.9890-9903.2004

FIG. 8.

FIG. 8.

Immunocytochemistry of cells transfected with truncation mutant vectors (MUT-8 to MUT-11). (A and B) Double staining of VP1 and VP2/VP3 (A) and of VP1 and agnoprotein (B) in cells transfected with MUT-8. (C) Double staining of VP1 and VP2/VP3 in cells transfected with MUT-9. (D and E) Double staining of VP1 and VP2/VP3 (D) and of VP1 and agnoprotein (E) in cells transfected with MUT-10. (F) Double staining of VP1 and VP2/VP3 in cells transfected with MUT-11. VP1 was visualized with Alexa fluor 488 (green), and VP2/VP3 or agnoprotein was visualized with Alexa fluor 568 (red). In panels D and F, truncated VP2/VP3 was not detected because of the deletion of the antigenic sequence for the anti-VP2/VP3C antibody.