FIG. 10.

Intracellular trafficking of Cy3-labeled Δ5/35 (red) and Cy2-labeled Ad5/35-β-gal vectors in MO7e cells. Cells were coinfected with indicated Ad vectors for 15 min and 2 h. Then, cells were fixed and stained to detect the colocalization of viruses within cellular endocytic compartments. Confocal images of cells were taken in both green and red channels. To confirm that both viruses labeled with different dyes are capable of entering the same endocytic compartments, MO7e cells were coinfected with Δ5/35 and Ad5/35-β-gal vectors (lower panels). Colocalization of two viruses appears as yellow. Representative fields are shown.