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First-generation capsid-modified Ad vector is unable to improve the infectivity of Δ5/35 vector on MO7e cells. MO7e cells were infected with Δ5/35 or Ad5/35 vectors expressing GFP at the indicated MOIs (virus particles per cell) together with increasing amounts of first-generation Ad5/35-β-galactosidase virus (FG). The percentage of GFP-expressing cells was analyzed at 24 h postinfection by flow cytometry (n = 4).
