FIG. 2.

NSP4 associates with the epithelial BM in mouse jejunum during rotavirus infection in neonatal mice. (A and C) With the anti-NSP4(114-135) rabbit polyclonal serum, NSP4 protein was detected in epithelial cells at the tips of intestinal villi at 4 dpi and at the epithelial BM of infected and noninfected cells (arrowheads). (B) Localization of NSP4 at the BM was not observed with preimmune serum. (D) NSP4 protein expression was not detected in control mice. (E) An antibody that recognizes rotavirus structural proteins stained infected epithelial cells but failed to stain the epithelial BMs. (F) NSP4 could occasionally be detected in the BMs of lower villus and crypt cells (arrowhead). Panels A and B are cross-sectional slides; magnification, ×400. Panels C to F are longitudinal slides; magnification, ×200 (C to E) and ×600 (F).