FIG. 3.

Colocalization of NSP4 with laminin-β3 in the BMs of small intestinal epithelial cells during rotavirus infection in neonatal mice. (A) Laminin-β3 was detected in the BMs of small intestinal epithelia at 4 dpi (arrow). (B) In the adjacent section, rotavirus-infected cells were detected with anti-NSP4 antibody (arrowhead), whereas the BMs of infected and noninfected cells were also stained (arrow). (C) Laminin-β3 was detected in the BM associated with the villus and crypt epithelium (open arrowhead) in 11-day-old mice. (D) In 28-day-old mice, laminin-β3 expression was decreased in the BM of the villus region, weakly present in the upper crypt region, and not observed in the lower crypt region (open arrowhead). Panels A and B are cross-sectional slides; magnification, ×600. Panels C and D are longitudinal slides; magnification, ×200.