TABLE 2.

Effects of CVB4 E2 on thymocyte subpopulations^a

Virus	% Live cellsb ± SD
	Live thymocytes	DP cells	SP4 cells	SP8 cells
Mock	58 ± 14	79 ± 7	5 ± 2	3 ± 1
CVB4 E2	26 ± 11	57 ± 12*	3 ± 2†	3 ± 1

^aMock- and CVB4 E2-infected FTOC were harvested 7 days after inoculation, and the distributions of live cells and thymocyte subpopulations were assessed by flow cytometry.

^bLive thymocytes, the percentage of live cells determined by 7-AAD exclusion staining; DP, SP4, and SP8, thymocyte subpopulations were quantified as a percentage of total live cells. SP4 and SP8 cells were gated based on high expression of CD3. Other cells include CD4⁻ CD8⁻, SP4 CD3^low, and SP8 CD3^low cells. *, P < 0.001 versus mock-infected thymocytes; †, P < 0.01 versus mock-infected thymocytes (n = 3).