FIG. 1.
Construction of GFP and RFP expressing BoHV-1. (a) Schematic representation of the BoHV-1 genome. Unique long (UL) and unique short (US) segments and the internal (IR) and terminal repeat (TR) sequences are marked. Arrows indicate isomerization of the US segment. (b) Location of the genes used for integration of GRP and RFP expression cassettes. The regions between nt 15007 and 18211 encoding gC and UL46 (partial) and between nt 119500 and 123501 encompassing genes for gD (partial), gI, and gE are enlarged. Arrowheads indicate the direction and termination of transcription, and black dots indicate transcription start sites. (c) Interruption of the gC ORF by the GFP expression cassette and replacement of the gI ORF by the RFP expression cassette. The exchanged sequences are indicated. Arrows mark the direction of GFP and RFP transcription. Cassettes are not drawn to scale. The numbering of nucleotides and location of the BoHV-1 genes correspond to the complete genome sequence (accession number AJ004801).