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TABLE 1.

Effect of salt on dissociation of M1 from RNPa

Strain M1/NP ratio in presence of NaCl (M)
% of M1 bound to RNP in presence of NaCl (M)
V 0.15 0.3 V 0.15 0.3
WT 1.45 1.25 0.93 100 86 64
C148S 1.33 1.21 0.62 100 91 47
R101S 0.74 0.52 0.30 100 70 40
R105S 0.71 0.47 0.28 100 64 39
R101S-R105S 0.42 0.30 0.05 100 71 12
a

The RNP-associated M1 and the ratio of M1 to NP were quantified by densitometry. The percentages of binding reactivities of M1s to RNP were calculated by comparisons of M1 protein to NP in virions and the M1/RNP ratio. The data are means of three independent experiments, and the standard deviation of each experimental point was below 10%. V, virion alone.