TABLE 1.
Strain | M1/NP ratio in presence of NaCl (M)
|
% of M1 bound to RNP in presence of NaCl (M)
|
||||
---|---|---|---|---|---|---|
V | 0.15 | 0.3 | V | 0.15 | 0.3 | |
WT | 1.45 | 1.25 | 0.93 | 100 | 86 | 64 |
C148S | 1.33 | 1.21 | 0.62 | 100 | 91 | 47 |
R101S | 0.74 | 0.52 | 0.30 | 100 | 70 | 40 |
R105S | 0.71 | 0.47 | 0.28 | 100 | 64 | 39 |
R101S-R105S | 0.42 | 0.30 | 0.05 | 100 | 71 | 12 |
The RNP-associated M1 and the ratio of M1 to NP were quantified by densitometry. The percentages of binding reactivities of M1s to RNP were calculated by comparisons of M1 protein to NP in virions and the M1/RNP ratio. The data are means of three independent experiments, and the standard deviation of each experimental point was below 10%. V, virion alone.