TABLE 2.
Monoclonal antibodies used to stain cell populations for FACS and flow cytometry
| Cell population stained | Fluorescent dye-conjugated mouse anti-human mono- clonal antibodya | Clone | Company |
|---|---|---|---|
| CD8 lymphocytes to be separated by differentiation phenotype and PBMCs for assessment of differentiation phenotype of CD8 lymphocytes expressing CD4 | CD27 FITC | M-T271 | BD Biosciences |
| CD8β chain PE | 2ST8.5H7 | Coulter Immunotech | |
| CD45RA Cy-chrome | HI100 | Pharmingen | |
| CD4-APC | RPA-T4 | BD Biosciences | |
| CD8 lymphocytes to be separated by CD4 expression | CD8β chain PE | 2ST8.5H7 | Coulter Immunotech |
| CD4 Cy-chrome | RPA-T4 | BD Biosciences | |
| CD4 lymphocytes | CD3 PE | UCHT1 | BD Biosciences |
| CD4 Cy-chrome | RPA-T4 | BD Biosciences |
a
FITC, fluorescein isothiocyanate isomer 1; PE, phycoerythrin; APC, allophycocyanin.