FIG. 1.

The surface expression of SLAM/CD150 is down regulated by measles virus infection. (A) Activated marmoset B-cell line B95-8 was infected with Montefiore measles virus. At 24 and 48 h p.i., SLAM surface expression was analyzed by FACS. (B) Marmoset B95-8 cells were infected with Edmonston measles virus. At 24 and 48 h p.i., SLAM surface expression was analyzed by FACS. (C) EBV-transformed human B-cell line 1A2 was infected with Montefiore measles virus. At 24 and 48 h p.i., SLAM expression was analyzed by FACS. (D) Human 1A2 cells were infected with Edmonston measles virus. At 24 and 48 h p.i., SLAM expression was determined by FACS. (E) Human 1A2 cells were infected with Montefiore measles virus. At 24 and 48 h p.i., CD46 expression was analyzed by FACS. (F) Human 1A2 cells were infected with Edmonston measles virus. At 24 and 48 h p.i., CD46 expression was analyzed by FACS. Grey lines, mock-infected cells stained with the anti-SLAM antibody (A to D) or anti-CD46 antibody (E and F) and detected with the FITC-conjugated goat anti-mouse antibody; black lines, mock-infected cells incubated with the FITC-conjugated goat anti-mouse secondary antibody only; solid peaks, cells infected with Montefiore (A, C, and E) or Edmonston (B, D, and F) measles virus stained with the anti-SLAM antibody (A to D) or the anti-CD46 antibody (E and F), followed by an FITC-conjugated goat anti-mouse antibody. Insets, levels of H protein expression on the surfaces of B95-8 and 1A2 cells infected with Montefiore 89 and Edmonston strains of measles virus following 48 h of incubation. The cells were stained with anti-measles H antibody, followed by FITC-conjugated goat anti-mouse secondary antibody. The solid lines represent infected cells; the dashed lines represent mock-infected cells.