FIG. 4.

pUL76 and H-pUL76 are associated with the virion envelope and tegument/capsid fractions, respectively. After treatment with detergents, virion proteins were fractionated into soluble supernatant and insoluble pellet, which were then resolved on an SDS-10% polyacrylamide gel. Lanes: 1, virion; 2, virion envelope proteins (Env) soluble in detergents; 3, virion tegument/capsid (T/C) proteins insoluble in detergents. (A) The protein compositions of the virions and fractions were visualized by silver staining. In parallel experiments, the proteins were subjected to Western blotting analyses with anti-pUL76 (B), anti-ppUL83 (C), and anti-gpUL55 (D) antibodies. As controls, gpUL55 and ppUL83, present in the envelope and tegument/capsid, respectively, were used to assess the purity of the fractions.